anti ddr2 rabbit polyclonal antibody  (Bioss)

Journal: Experimental and Therapeutic Medicine

Article Title: Allogeneic adipose-derived stem cell transplantation on knee osteoarthritis rats and its effect on MMP-13 and DDR2

doi: 10.3892/etm.2019.7554

Figure Lengend Snippet: DDR2 positive expression levels before and after treatment in the three groups of rats (%).

Article Snippet: Type II collagenase was purchased from Sigma; Merck KGaA (Darmstadt, Germany); rabbit anti-mouse DDR2 polyclonal antibody was purchased from ImmunoWay Biotechnology Company (Plano, TX, USA); TRIzol reagent was purchased from Invitrogen; Thermo Fisher Scientific, Inc. (Waltham, MA, USA); PCR fluorescence quantitative kit and reverse transcription kit were purchased from Dalian Takara Biotechnology Co., Ltd. (Dalian, China).

Techniques: Expressing, Transplantation Assay

Journal: Experimental and Therapeutic Medicine

Article Title: Allogeneic adipose-derived stem cell transplantation on knee osteoarthritis rats and its effect on MMP-13 and DDR2

doi: 10.3892/etm.2019.7554

Figure Lengend Snippet: Correlation analysis between MMP-13 mRNA and DDR2 in KOA rats. There was a positive correlation between MMP-13 mRNA and DDR2 in KOA rats (r=0.830, P<0.001). MMP-13, matrix metalloproteinase 13; DDR2, discoid domain receptor 2; KOA, knee osteoarthritis.

Article Snippet: Type II collagenase was purchased from Sigma; Merck KGaA (Darmstadt, Germany); rabbit anti-mouse DDR2 polyclonal antibody was purchased from ImmunoWay Biotechnology Company (Plano, TX, USA); TRIzol reagent was purchased from Invitrogen; Thermo Fisher Scientific, Inc. (Waltham, MA, USA); PCR fluorescence quantitative kit and reverse transcription kit were purchased from Dalian Takara Biotechnology Co., Ltd. (Dalian, China).

Techniques:

Journal: Scientific Reports

Article Title: Integrated Molecular Profiling of Human Gastric Cancer Identifies DDR2 as a Potential Regulator of Peritoneal Dissemination

doi: 10.1038/srep22371

Figure Lengend Snippet: ( a ) EEM-deduced activity profiles of GDES and other gene sets were evaluated by clustering analysis. ( b ) Kaplan–Meier survival curves for the 198 patients in the Singaporean cohort, with classification based on DDR2 mRNA expression levels measured using a microarray. ( c ) DDR2 expression was significantly higher in gastric cancer patients with peritoneal metastasis (Singaporean cohort). ( d ) Kaplan–Meier survival curves for the 195 patients in the Japanese cohort, as measured by qRT-PCR. ( e ) High DDR2 expression associated with gastric cancer patients that developed peritoneal metastasis, as determined by performing a Pearson’s chi-square test (Japanese cohort).

Article Snippet: For antigen retrieval, tissue sections were deparaffinized and boiled in 0.01 M sodium citrate buffer in a microwave for 10 minutes at 500 W. A rabbit polyclonal antibody against DDR2 (H-108, 1:100 dilution; Santa Cruz Biotechnology, Santa Cruz, CA) and a mouse monoclonal antibody against E-cadherin (36/E-Cadherin, 1:100 dilution; BD Bioscience) were used as primary antibodies.

Techniques: Activity Assay, Expressing, Microarray, Quantitative RT-PCR

Journal: Scientific Reports

Article Title: Integrated Molecular Profiling of Human Gastric Cancer Identifies DDR2 as a Potential Regulator of Peritoneal Dissemination

doi: 10.1038/srep22371

Figure Lengend Snippet: ( a ) Correlation plot between DDR2 expression and the fraction of methylation at the promoter region of DDR2 in 173 gastric cancer samples from the Singaporean cohort. ( b ) Box plot of the fraction of DDR2 promoter methylation between patients, with or without peritoneal metastasis. ( c ) Representative images of DDR2 and E-cadherin protein expression in gastric cancer cells. Scale bars, 100 μm. ( d) DDR2 and E-cadherin expression were inversely correlated by immunohistochemical analysis of 76 gastric cancer samples.

Article Snippet: For antigen retrieval, tissue sections were deparaffinized and boiled in 0.01 M sodium citrate buffer in a microwave for 10 minutes at 500 W. A rabbit polyclonal antibody against DDR2 (H-108, 1:100 dilution; Santa Cruz Biotechnology, Santa Cruz, CA) and a mouse monoclonal antibody against E-cadherin (36/E-Cadherin, 1:100 dilution; BD Bioscience) were used as primary antibodies.

Techniques: Expressing, Methylation, Immunohistochemical staining

Journal: Scientific Reports

Article Title: Integrated Molecular Profiling of Human Gastric Cancer Identifies DDR2 as a Potential Regulator of Peritoneal Dissemination

doi: 10.1038/srep22371

Figure Lengend Snippet: ( a ) DDR2 expression was suppressed in 58As9Luc cells by 2 independent shRNAs. ( b ) DDR2 knockdown resulted in lower cell proliferation on collagen I-coated 96-well plates, but did not affect cell proliferation on normal plates. ( c , d ) Effects of DDR2 on ( c ) cancer cell migration and ( d ) invasion. ( e ) DDR2-knockdown 58As9Luc cells were orthotopically transplanted into the stomach walls of nude mice. ( f , g ) The normalized weight of gastric wall tumors ( f ) and the number of disseminated metastatic tumors ( g ) at 28 days post-transplantation with 58As9Luc cells with silenced DDR2 expression. Control, DDR2 sh#1, and sh#2: n = 8. *p < 0.05, **p < 0.01.

Article Snippet: For antigen retrieval, tissue sections were deparaffinized and boiled in 0.01 M sodium citrate buffer in a microwave for 10 minutes at 500 W. A rabbit polyclonal antibody against DDR2 (H-108, 1:100 dilution; Santa Cruz Biotechnology, Santa Cruz, CA) and a mouse monoclonal antibody against E-cadherin (36/E-Cadherin, 1:100 dilution; BD Bioscience) were used as primary antibodies.

Techniques: Expressing, Knockdown, Migration, Transplantation Assay, Control